期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 97, 期 3, 页码 331-336出版社
SPRINGER
DOI: 10.1007/s11240-009-9518-x
关键词
Agrobacterium tumefaciens; Bactericidal activity; Phytotoxicity; Morphogenesis
资金
- International Foundation for Science (IFS)
- State of Bahia Research Foundation (FAPESB)
- National Council for Scientific and Technological Development (CNPq)
- Coordination of Higher Education and Graduate Training (CAPES)
Identification of beta-lactam antibiotics that have negligible effects on plant regeneration is a critical step towards the establishment of a reliable Agrobacterium-mediated transformation protocol for perennial trees. In the present report, we have evaluated the effects of the novel beta-lactam antibiotics meropenem and timentin on plant regeneration of a perennial woody fruit plant, Citrus sinensis, in comparison with the commonly used beta-lactam cefotaxime. It was observed that, in contrast to cefotaxime, meropenem and timentin had a positive or no detrimental effect on the shoot regeneration from epicotyl explants. Residual effects of the beta-lactams from shoot regeneration medium also affected the subsequent ability of the roots to elongate. The addition of meropenem and/or timentin in the rooting medium mostly improved or did not affect the rooting ability of the adventitious shoots. These data indicated that meropenem and timentin can positively replace cefotaxime in Agrobacterium-mediated transformation of C. sinensis.
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