期刊
PLANT CELL REPORTS
卷 33, 期 2, 页码 265-276出版社
SPRINGER
DOI: 10.1007/s00299-013-1527-x
关键词
Computational prediction; Expression analysis; MicroRNA; Sugarcane streak mosaic virus; Target genes
资金
- Department of Biotechnology, Government of India, New Delhi, India [BT/PR2402/AGR/36/698/2011]
Identification of miRNAs encoded by sugarcane streak mosaic virus and understanding their host target genes might be used as a new strategy to study viral pathogenesis and controlling mosaic disease in sugarcane. Plants have developed several defense mechanisms to cope with various pathogens (bacteria, fungi, virus, and phytoplasma). Among these, RNA interference (RNAi)-mediated defense against viral infection was found to be a major innate immune response. As a counter attack strategy against the host defense, viruses produce suppressors of host RNAi pathway. MicroRNAs (miRNAs) are an abundant class of short (similar to 18-22 nucleotide) non-coding single-stranded RNAs involved in RNAi pathway leading to post-transcriptional regulation of gene expression. Sugarcane streak mosaic virus (SCSMV) is a distinct strain of Potyviridae family which has a single-stranded positive-sense RNA genome causing mosaic disease in sugarcane. In this study, we computationally predicted and experimentally validated the miRNA encoded by the SCSMV genome with detection efficiency of 99.9 % in stem-loop RT-qPCR and predicted their potential gene targets in sugarcane. These sugarcane target genes considerably broaden future investigation of the SCSMV-encoded miRNA function during viral pathogenesis and might be applied as a new strategy for controlling mosaic disease in sugarcane.
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