期刊
PLANT CELL REPORTS
卷 31, 期 4, 页码 629-636出版社
SPRINGER
DOI: 10.1007/s00299-011-1179-7
关键词
Chitosanase; Chitosan; Disease resistance; Magnaporthe oryzae; Rice; MAMPs
资金
- Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry in Japan
- Grants-in-Aid for Scientific Research [22780044, 21380050, 21780064] Funding Source: KAKEN
Plant fungal pathogens change their cell wall components during the infection process to avoid degradation by host lytic enzymes, and conversion of the cell wall chitin to chitosan is likely to be one infection strategy of pathogens. Thus, introduction of chitosan-degradation activity into plants is expected to improve fungal disease resistance. Chitosanase has been found in bacteria and fungi, but not in higher plants. Here, we demonstrate that chitosanase, Cho1, from Bacillus circulans MH-K1 has antifungal activity against the rice blast fungus Magnaporthe oryzae. Introduction of the cho1 gene conferred chitosanase activity to rice cells. Transgenic rice plants expressing Cho1 designed to be localized in the apoplast showed increased resistance to M. oryzae accompanied by increased generation of hydrogen peroxide in the infected epidermal cells. These results strongly suggest that chitosan exists in the enzyme-accessible surface of M. oryzae during the infection process and that the enhancement of disease resistance is attributable to the antifungal activity of the secreted Cho1 and to increased elicitation of the host defense response.
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