期刊
PLANT CELL REPORTS
卷 29, 期 11, 页码 1215-1225出版社
SPRINGER
DOI: 10.1007/s00299-010-0907-8
关键词
Micropropagation; Disease resistance; Apple scab; Fruit quality; Shelf life; Transgenic; Cisgenic; Intragenic
资金
- University Grants Commission, Govt of India
- CSIR
Apple (Malus domestica Borkh.), which is a widely cultivated, important economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Apple cultivation is heavily dependent on climatic condition and is susceptible to several diseases caused by fungi, bacteria, viruses, insects, etc. Extensive research work has been carried out to standardize tissue culture protocols and utilize them in apple improvement. We review the in vitro shoot multiplication, rooting, transformation and regeneration methodologies in apple and tabulate various such protocols for easy reference. The utility and limitation of transgenesis in apple improvement have also been summarized. The concepts of marker-free plants, use of non-antibiotic resistance selectable markers, and cisgenic and intragenic approaches are highlighted. Furthermore, the limitations, current trends and future prospects of tissue culture-mediated biotechnological interventions in apple improvement are discussed.
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