期刊
PLANT CELL REPORTS
卷 29, 期 3, 页码 249-259出版社
SPRINGER
DOI: 10.1007/s00299-010-0818-8
关键词
Arabidopsis thaliana; Cycloheximide; Herbicide tolerance; Hygromycin B; L-Phosphinothricin; Proteolysis
资金
- Kentucky Tobacco Research and Development Center
- USDA Cooperative State Research, Education and Extension Service [20053530416043]
- Kentucky Science and Engineering Foundation [14850206189]
The 26S proteasome (26SP), the central protease of the ubiquitin-dependent proteolysis pathway, controls the regulated proteolysis of functional proteins and the removal of misfolded and damaged proteins. In Arabidopsis, cellular and stress response phenotypes of a number of mutants with partially impaired 26SP function have been reported. Here, we describe the responses of proteasome mutants to protein synthesis inhibitors. We show that the rpt2a-3, rpn10-1 and rpn12a-1 mutants are hypersensitive to the antibiotic hygromycin B, and tolerant to the translation inhibitor cycloheximide (CHX) and herbicide l-phosphinothricin (PPT). In addition to the novel mechanism for herbicide tolerance, our data suggests that the combination of hygromycin B, CHX and PPT growth-response assays could be used as a facile diagnostic tool to detect altered 26SP function in plant mutants and transgenic lines.
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