期刊
PLANT CELL REPORTS
卷 28, 期 2, 页码 233-239出版社
SPRINGER
DOI: 10.1007/s00299-008-0625-7
关键词
Cytoplasmic male sterility; Fertility restorer gene; Oryza sativa L.
资金
- Ministry of Education, Science, Sports and Culture, Japan [18075002]
- Ministry of Agriculture, Forestry and Fisheries of Japan [QT-2007]
- National Agricultural Research Center
- National Institute of Agrobiological Sciences
- Grants-in-Aid for Scientific Research [18075002] Funding Source: KAKEN
Rice with LD-type cytoplasmic male sterility (CMS) possesses the cytoplasm of 'Lead Rice' and its fertility is recovered by a nuclear fertility restorer gene Rf1. Rf1 promotes processing of a CMS-associated mitochondrial RNA of atp6-orf79, which consists of atp6 and orf79, in BT-CMS with the cytoplasm of 'Chinsurah Boro II'. In this study, we found that LD-cytoplasm contained a sequence variant of orf79 downstream of atp6. Northern blot analysis showed that atp6-orf79 RNA of LD-cytoplasm was co-transcribed and was processed in the presence of Rf1 in the same manner as in BT-cytoplasm. Western blot analysis showed that the ORF79 peptide did not accumulate in an LD-CMS line, while ORF79 accumulated in a BT-CMS line and was diminished by Rf1. These results suggest that accumulation of ORF79 is not the cause of CMS in LD-cytoplasm and the mechanism of male-sterility induction/fertility restoration in LD-CMS is different from that in BT-CMS.
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