期刊
PLANT CELL AND ENVIRONMENT
卷 37, 期 9, 页码 1989-2001出版社
WILEY-BLACKWELL
DOI: 10.1111/pce.12335
关键词
evolution; photosynthesis; stress
资金
- Plan Nacional, Spain [AGL2009-07999, BFU2011-23294]
- BBSRC [BBSRC BB/J/00426X/1, BBSRC BB/I002545/1, BB/I017372/1]
- Biotechnology and Biological Sciences Research Council [BB/I002545/1, BB/I017372/1, BBS/E/C/00004978, BBS/E/C/00005202] Funding Source: researchfish
- BBSRC [BB/I017372/1, BB/I002545/1, BBS/E/C/00004978, BBS/E/C/00005202] Funding Source: UKRI
The present study characterizes the kinetic properties of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from 28 terrestrial plant species, representing different phylogenetic lineages, environmental adaptations and photosynthetic mechanisms. Our findings confirm that past atmospheric CO2/O-2 ratio changes and present environmental pressures have influenced Rubisco kinetics. One evolutionary adaptation to a decreasing atmospheric CO2/O-2 ratio has been an increase in the affinity of Rubisco for CO2 (K-c falling), and a consequent decrease in the velocity of carboxylation (k(cat)(c)), which in turn has been ameliorated by an increase in the proportion of leaf protein accounted by Rubisco. The trade-off between K-c and k(cat)(c) was not universal among the species studied and deviations from this relationship occur in extant forms of Rubisco. In species adapted to particular environments, including carnivorous plants, crassulacean acid metabolism species and C-3 plants from aquatic and arid habitats, Rubisco has evolved towards increased efficiency, as demonstrated by a higher k(cat)(c)/K-c ratio. This variability in kinetics was related to the amino acid sequence of the Rubisco large subunit. Phylogenetic analysis identified 13 residues under positive selection during evolution towards specific Rubisco kinetic parameters. This crucial information provides candidate amino acid replacements, which could be implemented to optimize crop photosynthesis under a range of environmental conditions.
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