期刊
PLANT CELL AND ENVIRONMENT
卷 37, 期 9, 页码 2051-2063出版社
WILEY
DOI: 10.1111/pce.12285
关键词
symbiosis; proteome; sulfur metabolism
资金
- Spanish Ministry of Economy and Competitiveness [AGL 2011-23738, AGL 2011-30386-C02-01]
- Marie Curie International Outgoing Fellowship for Career Development [PIOF-GA-2009-253141]
- Public University of Navarre [735/2008]
Symbiotic nitrogen fixation is one of the first physiological processes inhibited in legume plants under water-deficit conditions. Despite the progress made in the last decades, the molecular mechanisms behind this regulation are not fully understood yet. Recent proteomic work carried out in the model legume Medicago truncatula provided the first indications of a possible involvement of nodule methionine (Met) biosynthesis and related pathways in response to water-deficit conditions. To better understand this involvement, the drought-induced changes in expression and content of enzymes involved in the biosynthesis of Met, S-adenosyl-L-methionine (SAM) and ethylene in M.truncatula root and nodules were analyzed using targeted approaches. Nitrogen-fixing plants were subjected to a progressive water deficit and a subsequent recovery period. Besides the physiological characterization of the plants, the content of total sulphur, sulphate and main S-containing metabolites was measured. Results presented here show that S availability is not a limiting factor in the drought-induced decline of nitrogen fixation rates in M.truncatula plants and provide evidences for a down-regulation of the Met and ethylene biosynthesis pathways in roots and nodules in response to water-deficit conditions.
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