期刊
PLANT CELL AND ENVIRONMENT
卷 36, 期 8, 页码 1449-1464出版社
WILEY
DOI: 10.1111/pce.12074
关键词
abscisic acid-; stress-; and ripening-induced (ASR) protein; antioxidant system; reactive oxygen species (ROS); stress-responsive genes
资金
- International S & T Cooperation Key Projects of MoST [2009DFB30340]
- National Genetically Modified New Varieties of Major Projects of China [2011ZX08002-004, 2011ZX08010-004]
- Key Projects of S & T Research of MoE of China [109105]
- Wuhan Municipal S & T research project [201120922286]
Abscisic acid (ABA)-, stress-, and ripening-induced (ASR) proteins are reported to be involved in abiotic stresses. However, it is not known whether ASR genes confer drought stress tolerance by utilizing the antioxidant system. In this study, a wheat ASR gene, TaASR1, was cloned and characterized. TaASR1 transcripts increased after treatments with PEG6000, ABA and H2O2. Overexpression of TaASR1 in tobacco resulted in increased drought/osmotic tolerance, which was demonstrated that transgenic lines had lesser malondialdehyde (MDA), ion leakage (IL) and reactive oxygen species (ROS), but higher relative water content (RWC) and superoxide dismutase (SOD) and catalase (CAT) activities than wild type (WT) under drought stress. Overexpression of TaASR1 in tobacco also enhanced the expression of ROS-related and stress-responsive genes under osmotic stress. In addition, transgenic lines exhibited improved tolerance to oxidative stress by retaining more effective antioxidant system. Finally, TaASR1 was localized in the cell nucleus and functioned as a transcriptional activator. Taken together, our results showed that TaASR1 functions as a positive factor under drought/osmotic stress, involved in the regulation of ROS homeostasis by activating antioxidant system and transcription of stress-associated genes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据