期刊
PLANT CELL AND ENVIRONMENT
卷 34, 期 12, 页码 2138-2148出版社
WILEY
DOI: 10.1111/j.1365-3040.2011.02410.x
关键词
aluminium resistance; tomato; transgenic; Xenopus oocytes
资金
- Zhejiang Science and Technology Bureau [2008C12005-2]
- Natural Science Foundation of China [31071849, 30821140538, 30625026]
- Changjiang Scholarship
- Natural Science Foundation of Zhejiang province [Y3090088]
- Fundamental Research Funds for Central Universities
Al-activated organic acid anion efflux from roots is an important Al resistance mechanism in plants. We have conducted homologous cloning and isolated Vigna umbellata multidrug and toxic compound extrusion (VuMATE), a gene encoding a de novo citrate transporter from rice bean. Al treatment up-regulated VuMATE expression in the root apex, but neither in the mature root region nor in the leaf. The degree of up-regulation of VuMATE was both partially Al concentration and time dependent, consistent with the delay in the onset of the Al-induced citrate efflux in rice bean roots. While La3+ moderately induced VuMATE expression, Cd2+ and Cu2+ did not induce the expression. Electrophysiological analysis of Xenopus oocytes expressing VuMATE indicated this transporter can mediate significant anion efflux across the plasma membrane. [14C]citrate efflux experiments in oocytes demonstrated that VuMATE is a H+-dependent citrate transporter. In addition, expression of VuMATE in transgenic tomato resulted in increased Al resistance, which correlated with an enhanced citrate efflux. Taken together, these findings suggest that VuMATE is a functional homolog of the known citrate transporters in sorghum, barley, maize and Arabidopsis. The similarities and differences of all the known citrate transporters associated with Al stress in the MATE family are also discussed.
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