期刊
PLANT CELL AND ENVIRONMENT
卷 33, 期 2, 页码 149-162出版社
WILEY
DOI: 10.1111/j.1365-3040.2009.02065.x
关键词
suppression subtractive hybridization; gene expression; signal transduction; reactive oxygen species; nitrogen sources; proline
资金
- National S&T Support Program for 11th Five-Year Plan [2006BAD01A1606, 2006BAD03A0104]
- China Postdoctoral Science Foundation Funded Project [20070410578]
- National '973' Project [2009CB119107]
- Forestry S&R Special Project of SFA [200804001]
gamma-aminobutyric acid (GABA) is a four-carbon non-protein amino acid presented in a wide range of organisms. In this study, a suppression subtractive hybridization (SSH) library was constructed using roots of a legume shrub, Caragana intermedia, with the combined treatment of 300 mM NaCl and 300 mM NaCl + 10 mM GABA. We obtained 224 GABA-regulated unique expressed sequence tags (ESTs) including signal transduction, transcriptional regulation, hormone biosynthesis, reactive oxygen species (ROS) and polyamine metabolism, etc. The key H2O2-generated genes, NADPH oxidase (CaGR60), peroxidase (CaGR61) and amine oxidase (CaGR62), were regulated at the mRNA level by 10 mM GABA, which clearly inhibited H2O2 accumulation brought about by NaCl stress in roots and leaves with the observation of 3,3'-diaminobenzidine (DAB) staining. Similarly, 10 mM GABA also regulated the expression of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) genes (CaGR30 and CaGR31) and ethylene production in NaCl-treated roots. Surprisingly, these H2O2-generated genes were enhanced at the mRNA level by a lower concentration of GABA, at 0.25 mM, but not other alternative nitrogen sources, and endogenous GABA accumulated largely just by the application of GABA at either concentration. Our results further proved that GABA, as a signal molecule, participates in regulating the expression of genes in plants under salt stress.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据