期刊
PLANT CELL AND ENVIRONMENT
卷 32, 期 8, 页码 1046-1059出版社
WILEY
DOI: 10.1111/j.1365-3040.2009.01987.x
关键词
drought stress; osmotic stress; oxidative stress; salt stress; stress-induced; thermotolerance
资金
- Program for New Century Excellent Talents in University [NCET-07-0201]
- Youth Science and Technology Phosphor Foundation of Shanghai [08QH14003]
- Science and Technology Foundation of Shanghai [083391910400]
- National Talent Training Fund in Basic Research of China [J0630643]
Among the heat shock proteins (HSPs) of higher plants, those belonging to the small HSP (sHSP) family remain the least characterized in functional terms. To improve our understanding of sHSPs, we have characterized RcHSP17.8 from Rosa chinensis. Sequence alignments and phylogenetic analysis reveal this to be a cytosolic class I sHSP. RcHSP17.8 expression in R. chinensis was induced by heat, cold, salt, drought, osmotic and oxidative stresses. Recombinant RcHSP17.8 was overexpressed in Escherichia coli and yeast to study its possible function under stress conditions. The recombinant E. coli and yeast cells that accumulated RcHSP17.8 showed improved viability under thermal, salt and oxidative stress conditions compared with control cultures. We also produced transgenic Arabidopsis thaliana that constitutively expressed RcHSP17.8. These plants exhibited increased tolerance to heat, salt, osmotic and drought stresses. These results suggest that R. chinensis cytosolic class I sHSP (RcHSP17.8) has the ability to confer stress resistance not only to E. coli and yeast but also to plants grown under a wide variety of unfavorable environmental conditions.
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