期刊
PLANT CELL AND ENVIRONMENT
卷 32, 期 7, 页码 837-850出版社
WILEY
DOI: 10.1111/j.1365-3040.2009.01962.x
关键词
jasmonic acid; phosphatidic acid; PLD
资金
- EU ROST [QLK5-CT-2002-00841]
- Netherlands Organization for Scientific Research (NWO) [813.06.0039, 863.04.004, 864.05.001]
- European Union [FA0605]
- Royal Netherlands Academy of Arts and Sciences
Plants respond to wounding by means of a multitude of reactions, with the purpose of stifling herbivore assault. Phospholipase D (PLD) has previously been implicated in the wounding response. Arabidopsis (Arabidopsis thaliana) AtPLD alpha 1 has been proposed to be activated in intact cells, and the phosphatidic acid (PA) it produces to serve as a precursor for jasmonic acid (JA) synthesis and to be required for wounding-induced gene expression. Independently, PLD activity has been reported to have a bearing on wounding-induced MAPK activation. However, which PLD isoforms are activated, where this activity takes place (in the wounded or non-wounded cells) and what exactly the consequences are is a question that has not been comprehensively addressed. Here, we show that PLD activity during the wounding response is restricted to the ruptured cells using P-32(i)-labelled phospholipid analyses of Arabidopsis pld knock-out mutants and PLD-silenced tomato cell-suspension cultures. pld alpha 1 knock-out lines have reduced wounding-induced PA production, and the remainder is completely eliminated in a pld alpha 1/delta double knock-out line. Surprisingly, wounding-induced protein kinase activation, AtLOX2 gene expression and JA biosynthesis were not affected in these knock-out lines. Moreover, larvae of the Cabbage White butterfly (Pieris rapae) grew equally well on wild-type and the pld knock-out mutants.
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