期刊
PLANT CELL
卷 24, 期 11, 页码 4731-4747出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.112.106039
关键词
-
资金
- Natural Science Foundation of China [30830076]
- Changjiang Scholarship
- Program for Innovative Research Team in Universities [IRT1185]
- Fundamental Research Funds for the Central Universities
- UK Biotechnology and Biological Sciences Research Council
- U.S. National Science Foundation [MCB 0817976]
- Biotechnology and Biological Sciences Research Council [BB/E013651/1] Funding Source: researchfish
- BBSRC [BB/E013651/1] Funding Source: UKRI
Xyloglucan endohydrolase (XEH) and xyloglucan endotransglucosylase (XET) activities, encoded by xyloglucan endotransglucosylase-hydrolase (XTH) genes, are involved in cell wall extension by cutting or cutting and rejoining xyloglucan chains, respectively. However, the physiological significance of this biochemical activity remains incompletely understood. Here, we find that an XTH31 T-DNA insertion mutant, xth31, is more Al resistant than the wild type. XTH31 is bound to the plasma membrane and the encoding gene is expressed in the root elongation zone and in nascent leaves, suggesting a role in cell expansion. XTH31 transcript accumulation is strongly downregulated by Al treatment. XTH31 expression in yeast yields a protein with an in vitro XEH: XET activity ratio of >5000:1. xth31 accumulates significantly less Al in the root apex and cell wall, shows remarkably lower in vivo XET action and extractable XET activity, has a lower xyloglucan content, and exhibits slower elongation. An exogenous supply of xyloglucan significantly ameliorates Al toxicity by reducing Al accumulation in the roots, owing to the formation of an Al-xyloglucan complex in the medium, as verified by an obvious change in chemical shift of Al-27-NMR. Taken together, the data indicate that XTH31 affects Al sensitivity by modulating cell wall xyloglucan content and Al binding capacity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据