期刊
PLANT CELL
卷 24, 期 2, 页码 415-427出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.111.094144
关键词
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资金
- Kentucky Tobacco Research and Development Center
- USDA-National Research Initiative [2006-35301-17115, 2006-35100-17433]
- National Science Foundation [MCB-0718029, Subaward S-00000260, IOS-1048216]
- Kentucky Science and Technology Corporation [KSTC-144-401-08-029]
- Michigan Tech University
- National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases [K01 DK078648-01, R03 DK084166-01]
- Direct For Biological Sciences [1021465] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1219316] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [1021465] Funding Source: National Science Foundation
MicroRNAs (miRNAs) and other endogenous small RNAs act as sequence-specific regulators of the genome, transcriptome, and proteome in eukaryotes. The interrogation of small RNA functions requires an effective, widely applicable method to specifically block small RNA function. Here, we report the development of a highly effective technology that targets specific endogenous miRNAs or small interfering RNAs for destruction in Arabidopsis thaliana. We show that the expression of a short tandem target mimic (STTM), which is composed of two short sequences mimicking small RNA target sites, separated by a linker of an empirically determined optimal size, leads to the degradation of targeted small RNAs by small RNA degrading nucleases. The efficacy of the technology was demonstrated by the strong and specific developmental defects triggered by STTMs targeting three miRNAs and an endogenous siRNA. In summary, we developed an effective approach for the destruction of endogenous small RNAs, thereby providing a powerful tool for functional genomics of small RNA molecules in plants and potentially animals.
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