期刊
PLANT CELL
卷 23, 期 2, 页码 515-533出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.110.074369
关键词
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资金
- National Key Basic Research Developments Program
- Ministry of Science and Technology, P.R. China [2009CB941500, 2006CB101700]
- National 863 High-Tech Project [2006AA10A102]
- National Natural Science Foundation of China [30725022, 30600347]
- Shanghai Leading Academic Discipline Project [B205]
- National Transgenic Major Program [2011ZX08009-003-003]
The rice (Oryza sativa) floral homeotic C-class gene, MADS3, was previously shown to be required for stamen identity determination during early flower development. Here, we describe a role for MADS3 in regulating late anther development and pollen formation. Consistent with this role, MADS3 is highly expressed in the tapetum and microspores during late anther development, and a newly identified MADS3 mutant allele, mads3-4, displays defective anther walls, aborted microspores, and complete male sterility. During late anther development, mads3-4 exhibits oxidative stress-related phenotypes. Microarray analysis revealed expression level changes in many genes in mads3-4 anthers. Some of these genes encode proteins involved in reactive oxygen species (ROS) homeostasis; among them is MT-1-4b, which encodes a type 1 small Cys-rich and metal binding protein. In vivo and in vitro assays showed that MADS3 is associated with the promoter of MT-1-4b, and recombinant MT-1-4b has superoxide anion and hydroxyl radical scavenging activity. Reducing the expression of MT-1-4b causes decreased pollen fertility and an increased level of superoxide anion in transgenic plants. Our findings suggest that MADS3 is a key transcriptional regulator that functions in rice male reproductive development, at least in part, by modulating ROS levels through MT-1-4b.
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