期刊
PLANT CELL
卷 23, 期 7, 页码 2738-2753出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.111.086975
关键词
-
资金
- National Science Foundation [IOS-0923312]
- Florida Agricultural Experiment Station
- Centre of Biosystems Genomics
- Netherlands Metabolomics Centre that are part of the Netherlands Genomic Initiative
- Alexander von Humboldt scholarship
- Max-Planck-Society
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [0923312] Funding Source: National Science Foundation
Altering expression of transcription factors can be an effective means to coordinately modulate entire metabolic pathways in plants. It can also provide useful information concerning the identities of genes that constitute metabolic networks. Here, we used ectopic expression of a MYB transcription factor, Petunia hybrida ODORANT1, to alter Phe and phenylpropanoid metabolism in tomato (Solanum lycopersicum) fruits. Despite the importance of Phe and phenylpropanoids to plant and human health, the pathway for Phe synthesis has not been unambiguously determined. Microarray analysis of ripening fruits from transgenic and control plants permitted identification of a suite of coregulated genes involved in synthesis and further metabolism of Phe. The pattern of coregulated gene expression facilitated discovery of the tomato gene encoding prephenate aminotransferase, which converts prephenate to arogenate. The expression and biochemical data establish an arogenate pathway for Phe synthesis in tomato fruits. Metabolic profiling and C-13 flux analysis of ripe fruits further revealed large increases in the levels of a specific subset of phenylpropanoid compounds. However, while increased levels of these human nutrition-related phenylpropanoids may be desirable, there were no increases in levels of Phe-derived flavor volatiles.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据