期刊
PLANT CELL
卷 23, 期 4, 页码 1249-1263出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.110.082537
关键词
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资金
- Bundesministerium fur Bildung und Forschung [0314000]
- European Union commission [FP7-212019]
- Ministry of Education, Youth, and Sports of the Czech Republic
- European Regional Development Fund [CZ.1.05/2.1.00/01.0007]
- Grants-in-Aid for Scientific Research [23380007] Funding Source: KAKEN
We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign; similar to 86% of the estimated; similar to 32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
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