期刊
PLANT CELL
卷 21, 期 10, 页码 3170-3184出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.109.069179
关键词
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资金
- Centre National de la Recherche Scientifique
- Genoplante [ABRUPT/GNP05037G]
- Ministerio de Educacion y Ciencia and Fondo Europeo de Desarrollo Regional [BIO2008-00221]
- Marie-Curie early-stage training fellowship ADONIS [MEST-CT-2005-020232]
- Consejo Superior de Investigaciones Cientificas
- french Ministere de l'Enseignement Supe rieur et de la Recherche
The plant hormone abscisic acid (ABA) orchestrates plant adaptive responses to a variety of stresses, including drought. This signaling pathway is regulated by reversible protein phosphorylation, and genetic evidence demonstrated that several related protein phosphatases 2C (PP2Cs) are negative regulators of this pathway in Arabidopsis thaliana. Here, we developed a protein phosphatase profiling strategy to define the substrate preferences of the HAB1 PP2C implicated in ABA signaling and used these data to screen for putative substrates. Interestingly, this analysis designated the activation loop of the ABA activated kinase OST1, related to Snf1 and AMPK kinases, as a putative HAB1 substrate. We experimentally demonstrated that HAB1 dephosphorylates and deactivates OST1 in vitro. Furthermore, HAB1 and the related PP2Cs ABI1 and ABI2 interact with OST1 in vivo, and mutations in the corresponding genes strongly affect OST1 activation by ABA. Our results provide evidence that PP2Cs are directly implicated in the ABA-dependent activation of OST1 and further suggest that the activation mechanism of AMPK/Snf1-related kinases through the inhibition of regulating PP2Cs is conserved from plants to human.
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