期刊
PLANT CELL
卷 20, 期 12, 页码 3273-3288出版社
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.107.057489
关键词
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资金
- Japan Society for the Promotion of Science [17570031, 18657017]
- Ministry of Education, Culture, Sports, Science and Technology [17054029]
- Hayashi Memorial Foundation for Female Natural Scientists
- Asahi Glass Foundation
- Grants-in-Aid for Scientific Research [17054029, 17570031, 18657017] Funding Source: KAKEN
The homeostasis of gibberellins (GAs) is maintained by negative feedback in plants. REPRESSION OF SHOOT GROWTH (RSG) is a tobacco (Nicotiana tabacum) transcriptional activator that has been suggested to play a role in GA feedback by the regulation of GA biosynthetic enzymes. The 14-3-3 signaling proteins negatively regulate RSG by sequestering it in the cytoplasm in response to GAs. The phosphorylation on Ser-114 of RSG is essential for 14-3-3 binding of RSG. Here, we identified tobacco Ca2+- dependent protein kinase (CDPK1) as an RSG kinase that promotes 14-3-3 binding to RSG by phosphorylation of Ser-114 of RSG. CDPK1 interacts with RSG in a Ca2+- dependent manner in vivo and in vitro and specifically phosphorylates Ser-114 of RSG. Inhibition of CDPK repressed the GA-induced phosphorylation of Ser-114 of RSG and the GA-induced nuclear export of RSG. Overexpression of CDPK1 inhibited the feedback regulation of a GA 20-oxidase gene and resulted in sensitization to the GA biosynthetic inhibitor. Our results suggest that CDPK1 decodes the Ca2+ signal produced by GAs and regulates the intracellular localization of RSG.
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