期刊
PLANT BREEDING
卷 132, 期 6, 页码 563-570出版社
WILEY
DOI: 10.1111/pbr.12086
关键词
food barley; low temperature tolerance; marker assisted selection; betaglucan; vernalization; winter barley
资金
- USDA-CSREES-NRI [2006-55606-16722]
- Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2009-85606-05701]
- NIFA [2009-85606-05701, 581778] Funding Source: Federal RePORTER
Barley (Hordeum vulgare) is an important component of heart-healthy whole grain diets because it contains -glucan. All current US barley varieties with high -glucan are spring habit and have waxy starch. Winter varieties have agronomic advantages but require low-temperature tolerance (LTT). Vernalization sensitivity (VS) is associated with higher levels of LTT. To rapidly develop fall-sown varieties with LTT and higher grain -glucan, we therefore used marker-assisted selection (MAS) at the WX and VRN-H2 loci. The MAS-derived lines, together with unrelated non-waxy germplasm developed via phenotypic selection (PS), were used for a genome-wide association scan (GWAS). The panel was phenotyped for grain -glucan, LTT and VS. It was genotyped with 3072 single-nucleotide polymorphisms (SNPs) and allele-specific primers. Marker-assisted selection fixed target alleles at both loci but only one of the target phenotypes (higher -glucan percentage) was achieved. Variation for VS and LTT is attributable to (i) incomplete information about VRN-H1 at the outset of the project and (ii) unexpected allelic variation at VRN-H3 with a large effect on VS and LTT.
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