期刊
PLANT BREEDING
卷 131, 期 4, 页码 540-544出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1439-0523.2012.01978.x
关键词
marker-assisted selection; Lupinus angustifolius; sequence-specific marker; microsatellite-anchored fragment length polymorphisms; non-shattering pod
资金
- Grain Research and Development Corporation (Australia) [DAW0170]
- China Scholarship Council-The University of Western Australia Joint PhD Scholarship
With 1 figure and 4 tables Abstract Marker-assisted selection (MAS) is desirable for breeding non-shattering pods in narrow-leafed lupin and for facilitating the process of broadening the gene pool of existing domesticated Australian lupin cultivars. In this study, we developed a molecular marker using a strategy to incorporate the validation step during the candidate marker identification step by microsatellite-anchored fragment length polymorphisms (MFLP). Three MFLP polymorphisms showing banding patterns consistent with pod-shattering phenotypes on the 12 representative F8 recombinant inbred lines (RILs) were identified as candidate markers linked to lentus. One of these three markers matching the lentus phenotypes on representative cultivars and wild accessions was selected and converted into a co-dominant PCR-based marker, named LeLi. When marker LeLi was applied to 25 cultivars released in Australia and 125 wild core accessions of the Australian Lupin Collection, an overall matching rate of 60.67% was found assuming correct determination of the phenotypes. This newly developed marker is simple PCR-based and co-dominant and can be used for MAS in current lupin breeding in Western Australia.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据