Simple sequence repeats (SSRs) in faba bean: new loci from Orobanche-resistant cultivar 'Giza 402'

Genomic DNA from an inbred line from the Orobanche crenata resistant cultivar 'Giza 402' was restricted and enriched for AG-containing simple sequence repeats (SSRs). The overall SSR enrichment rate was 54.6%. Of 73 selected SSR markers that successfully amplified in 'Giza 402' 54 were polymorphic among a group of 10 Egyptian and Spanish faba bean genotypes extensively used in Orobanche crenata resistance/susceptibility studies and linkage map development. The polymorphism information content ranged from 0.16 to 0.72 and Unweighted Pair-Group Method using Arithmetic averages cluster analysis based on these loci placed all resistant genotypes descended from the parental line F 402 in the same group. Successful amplification from 28 primer pairs was also observed in five European faba bean genotypes. The SSR loci described here will be extremely useful in studies focusing on Orobanche crenata resistance, genetic diversity studies and will provide additional co-dominant loci to complement existing faba bean linkage maps based primarily on dominant markers.