期刊
PLANT BIOTECHNOLOGY REPORTS
卷 6, 期 1, 页码 69-75出版社
SPRINGER
DOI: 10.1007/s11816-011-0201-4
关键词
Potato; 3GT gene; Anthocyanin; Transformation
资金
- Opening Foundation of Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake [HZHL0807]
In order to explore a biotechnological method for improving potato tuber color and creating plants with increased anthocyanin contents, a potato UDP-glucose: flavonoid-3-O-glucosyltransferase (3GT) gene was inserted behind the GBSSI promoter of pBin19, and this construct was introduced into Solanum tuberosum L. cultivar D,sir,e plants by Agrobacterium-mediated transformation. Six independent transgenic lines overexpressing the 3GT gene were identified by PCR and Southern blot analysis from 18 kanamycin-resistant plants. Due to the expression of 3GT gene, the tuber color and the anthocyanin content were enhanced noticeably in the transgenic plants compared to the wild-type control plants. This result suggests that the 3GT gene can potentially be used to improve potato color and enhance levels of antioxidants in the diet.
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