期刊
PLANT BIOTECHNOLOGY REPORTS
卷 3, 期 2, 页码 127-134出版社
SPRINGER
DOI: 10.1007/s11816-009-0081-z
关键词
OsNAC; NAC transcription factor; Heterodimer; Homodimer; Yeast two hybrid
资金
- Ministry of Education, Science and Technology, Korea [CG2111]
- Biogreen21
Members of the NAM-ATAF-CUC (NAC) protein family are plant-specific transcription factors that contain a highly conserved N-terminal NAC-domain and diverse C-terminal regions. They have been implicated in plant development and abiotic stress responses. To identify interacters of rice NAC-domain proteins (OsNACs), we performed yeast two-hybrid screening of rice cDNA library using OsNAC5 as a bait, and the results showed that OsNAC5 interacts with other OsNACs including itself. To delineate an interacting domain, a series of deletion constructs of four OsNACs were made and transformed into yeast in various combinations. The results revealed that the conserved NAC domain of OsNACs plays a primary role in homodimer and heterodimer formation, and a part of C-terminal sequence is also necessary for the interaction. In vitro pull-down assays using recombinant OsNAC proteins verified the dimer formations, together suggesting that OsNACs may act by forming homodimers and/or heterodimers in plants.
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