期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 12, 期 6, 页码 787-796出版社
WILEY
DOI: 10.1111/pbi.12183
关键词
single nucleotide polymorphism; polyploid wheat; wheat iSelect array; genotyping; high-density map; genetic diversity
资金
- USDA AFRI Triticeae-CAP USDA AFRI [2011-68002-30029, 2009-65300-05638]
- Borlaug Global Rust Initiative
- National Science Foundation [DBI-0701916]
- Department of Primary Industries of Victoria
- Grains Research and Development Corporation Australia
- Howard Hughes Medical Institute
- Gordon & Betty Moore Foundation
- CSIRO Food Futures Flagship
- Agroalimentare e ricerca
- Genome Canada
- Genome Prairie
- Province of Saskatchewan
- Western Grains Research Foundation
- BBSRC WISP [BB/I003207/1]
- BBSRC [BB/I003207/1, BB/F010370/1, BB/E01268X/1, BB/F001185/1, BB/L014106/1, BB/I017496/1, BB/L020718/1, BB/I01750X/1, BB/E000126/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I01750X/1, BB/E01268X/1, BB/F010370/1, BBS/B/01839, BBS/B/02118, BB/L014106/1, BB/L020718/1, BB/F001185/1, EGA17701, BB/E000126/1, BBS/B/07446, BB/I017496/1, BB/I003207/1] Funding Source: researchfish
- ARS [813434, ARS-0424595] Funding Source: Federal RePORTER
High-density single nucleotide polymorphism (SNP) genotyping arrays are a powerful tool for studying genomic patterns of diversity, inferring ancestral relationships between individuals in populations and studying marker-trait associations in mapping experiments. We developed a genotyping array including about 90 000 gene-associated SNPs and used it to characterize genetic variation in allohexaploid and allotetraploid wheat populations. The array includes a significant fraction of common genome-wide distributed SNPs that are represented in populations of diverse geographical origin. We used density-based spatial clustering algorithms to enable high-throughput genotype calling in complex data sets obtained for polyploid wheat. We show that these model-free clustering algorithms provide accurate genotype calling in the presence of multiple clusters including clusters with low signal intensity resulting from significant sequence divergence at the target SNP site or gene deletions. Assays that detect low-intensity clusters can provide insight into the distribution of presence-absence variation (PAV) in wheat populations. A total of 46 977 SNPs from the wheat 90K array were genetically mapped using a combination of eight mapping populations. The developed array and cluster identification algorithms provide an opportunity to infer detailed haplotype structure in polyploid wheat and will serve as an invaluable resource for diversity studies and investigating the genetic basis of trait variation in wheat.
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