期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 11, 期 6, 页码 681-690出版社
WILEY
DOI: 10.1111/pbi.12058
关键词
HIV-1; enveloped virus-like particles; transgenic plants; transient expression; Gag; gp41
资金
- National Institutes of Health [U19 AI062150, U54 GM094599, R03 AI073157]
- United States Army Medical Research and Materiel Command
- Telemedicine and Advanced Technology Research Center [W81XWH-09-2-0022]
The transmembrane HIV-1 envelope protein gp41 has been shown to play critical roles in the viral mucosal transmission and infection of CD4+ cells. Gag is a structural protein configuring the enveloped viral particles and has been suggested to constitute a target of the cellular immunity that may control viral load. We hypothesized that HIV enveloped virus-like particles (VLPs) consisting of Gag and a deconstructed form of gp41 comprising the membrane proximal external, transmembrane and cytoplasmic domains (dgp41) could be expressed in plants. To this end, plant-optimized HIV-1 genes were constructed and expressed in Nicotiana benthamiana by stable transformation, or transiently using a Tobamovirus-based expression system or a combination of both. Our results of biophysical, biochemical and electron microscopy characterization demonstrates that plant cells could support not only the formation of enveloped HIV-1 Gag VLPs, but also the accumulation of VLPs that incorporated dgp41. These findings provide further impetus for the journey towards a broadly efficacious and inexpensive subunit vaccine against HIV-1.
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