期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 8, 期 2, 页码 196-210出版社
WILEY
DOI: 10.1111/j.1467-7652.2009.00477.x
关键词
Insertion sire-based polymorphism; molecular marker; polymorphism; mapping; market-assisted selection; wheat
资金
- Agence Nationale de la Recherche-Direction Regionale de la Recherche et de la Technologie [06-CPER-054-01]
- INRA
- Fonds Unique Interministeriel
In wheat, the deployment of marker-assisted selection has long been hampered by the lack of markers compatible with high-throughput cost-effective genotyping techniques Recently, insertion site-based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS flexible and high-throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci: and high level of polymorphism in breeding material Toward this aim, we developed a programme, IsbpFinder for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot(R) Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP-derived SNP markers We demonstrate that the high level of polymorphism of the ISBPs combined with cost-effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker-assisted selection
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据