期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 8, 期 9, 页码 994-1004出版社
WILEY
DOI: 10.1111/j.1467-7652.2010.00526.x
关键词
seed bioreactor; malaria vaccine; Plasmodium falciparum
资金
- UGC-HKSAR
- Hong Kong RGC [CUHK 4580/05M]
Malaria is widely associated with poverty, and a low-cost vaccine against malaria is highly desirable for implementing comprehensive vaccination programmes in developing countries. Production of malaria antigens in plants is a promising approach, but its development has been hindered by poor expression of the antigens in plant cells. In the present study, we targeted plant seeds as a low-cost vaccine production platform and successfully expressed the Plasmodium falciparum 42-kDa fragment of merozoite surface protein 1 (MSP1(42)), a leading malaria vaccine candidate, at a high level in transgenic Arabidopsis seeds. We overcame hurdles of transcript and protein instabilities of MSP1(42) in plants by synthesizing a plant-optimized MSP1(42) cDNA and either targeting the recombinant protein to protein storage vacuoles or fusing it with a stable plant storage protein. An exceptional improvement in MSP1(42) expression, from an undetectable level to 5% of total extractable protein, was achieved with these combined strategies. Importantly, the plant-derived MSP1(42) maintains its natural antigenicity and can be recognized by immune sera from malaria-infected patients. Our results provide a strong basis for the development of a plant-based, low-cost malaria vaccine.
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