4.2 Article

Microscale thioacidolysis method for the rapid analysis of β-O-4 substructures in lignin

期刊

PLANT BIOTECHNOLOGY
卷 29, 期 4, 页码 419-423

出版社

JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
DOI: 10.5511/plantbiotechnology.12.0627a

关键词

High-throughput; lignin; microscale; thioacidolysis; beta-O-4 substructure

资金

  1. Research and development of genetic and metabolic information of energy producing plants for breeding programs from the New Energy and Industrial Technology Development Organization (NEDO)
  2. Japan Society for Promotion of Science [20380102, 23658144]
  3. Grants-in-Aid for Scientific Research [23658144, 20380102] Funding Source: KAKEN

向作者/读者索取更多资源

Thioacidolysis is a method to detect the beta-O-4 substructures of lignin, and has been employed as a diagnostic test for the presence of lignin. However, the conventional thioacidolysis protocol is low-throughput and is a bottleneck in the characterization of lignins in a large number of samples such as transgenic lines. Recently, a rapid analysis protocol for thioacidolysis was reported. In this study, we modified mainly the work-up process. Our microscale protocol showed higher yields of thioacidolysis products than the conventional protocol.

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