期刊
PLANT BIOSYSTEMS
卷 150, 期 2, 页码 236-243出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/11263504.2014.969355
关键词
essential oils; Mentha hybrids; PCR-RFLP; molecular discrimination; 5S rRNA NTS
资金
- Doctorate of Pharmaceutical and Biomolecular Sciences of the University of Turin
Hybridization of species belonging to the genus Mentha is quite common. However, the indicators of hybridity are many and make Mentha hybrids' identification difficult. By using the same molecular strategy that allowed us to unequivocally identify some Mentha species, we amplified the Not-Transcribed-Spacer (NTS) of the 5S-rRNA gene to characterize the industrial crop peppermint, M.xpiperita and some important Mentha interspecific hybrids: M.xdalmatica, M.xdumetorum, M. xrotundifolia, M.xmaximilianea, M.xsmithiana, M.xverticillata, M.xvillosa. DNA amplification, sequence and cluster analysis revealed differences in the 5S-rRNA NTS region of Mentha hybrids. Peppermint and all other hybrids were unequivocally discriminated by RFLP analysis by using TaqI restriction enzyme, while a further discrimination between M. xdumetorum and M.xverticillata was obtained by XhoI restriction enzyme. Essential oil composition showed clustering patterns similar to DNA fingerprint, with a clear discrimination between plants producing menthofuran (e.g. M. aquatica and its related hybrids, including peppermint) and those containing piperitenone oxide (M. longifolia and its related hybrids).
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