期刊
PLANT AND SOIL
卷 370, 期 1-2, 页码 593-604出版社
SPRINGER
DOI: 10.1007/s11104-013-1656-6
关键词
Heavy metals; Dark septate endophytes; Salix caprea; Transpiration; Chlorophyll
Dark septate endophyte (DSE) fungi are the most frequent colonists on the roots of Salix caprea in highly metal-contaminated soils. The present study was performed to obtain insights into the physiology and potential role(s) of DSE fungi for Salix caprea growing in metal-enriched sites. Fungal isolates from S. caprea roots were identified using molecular methods, and tested for their tolerance and metal accumulation in axenic cultures. In addition, an inoculation experiment was performed to monitor the effects of the fungi on the metal uptake by the plant host. Fitness of S. caprea cuttings was assessed by photosynthetic pigment quantification and measurements of transpiration. Fungal isolates were identified as members of the Phialophora/Cadophora complex. They showed different levels of metal tolerance and maintained growth on metal-enriched media. In comparison to non-inoculated cuttings, the DSE-inoculated cuttings had lower leaf Cd content, and for isolates DB146 and DB148, also lower Zn concentrations. All DSE isolates increased the chlorophyll levels of the cuttings, with isolate DB146 also positively affecting the transpiration rate of S. caprea. Our findings suggest that DSE reduced the metal uptake by the S. caprea cuttings, thus suggesting a beneficial role for S. caprea in metal-enriched soils.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据