期刊
PLANT AND SOIL
卷 369, 期 1-2, 页码 509-513出版社
SPRINGER
DOI: 10.1007/s11104-013-1599-y
关键词
Carbon; Fourier-transform infrared spectroscopy; Grinding; Microcentrifuge tubes; Milling; Nitrogen
资金
- Biotechnology and Biological Sciences Research Council
Milling of plant and soil material in plastic tubes, such as microcentrifuge tubes, over-estimates carbon (C) and under-estimates nitrogen (N) concentrations due to the introduction of polypropylene into milled samples, as identified using Fourier-transform infra-red spectroscopy. This study compares C and N concentrations of roots and soil milled in microcentrifuge tubes versus stainless steel containers, demonstrating that a longer milling time, greater milling intensity, smaller sample size and inclusion of abrasive sample material all increase polypropylene contamination from plastic tubes leading to overestimation of C concentrations by up to 8 % (0.08 g g(-1)). Erroneous estimations of C and N, and other analytes, must be assumed after milling in plastic tubes and milling methods should be adapted to minimise such error.
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