期刊
PLANT AND CELL PHYSIOLOGY
卷 56, 期 4, 页码 790-802出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcv007
关键词
Alkaline stress; GSNOR; RNS; ROS; S-nitrosylation; Tomato
资金
- National Natural Science Foundation of China [31372059]
- Agricultural Biology Resource Innovation Project, Breeding Program of Shandong Province, China [PTBR2013]
- Ministry of Science and Technology of China [National Key Technology RD Program] [2013BAD01B04-16]
- Excellent Young Scientist Foundation of Shandong Province [BS2014NY005]
S-Nitrosoglutathione reductase (GSNOR) plays an important role in regulating nitric oxide (NO) and S-nitrosothiol (SNO) homeostasis, and is therefore involved in the modulation of processes mediated by reactive nitrogen species (RNS). Although RNS have emerged as a key component in plant response to abiotic stress, knowledge of their regulation by GSNOR under alkaline stress was lacking. In this study, metabolic regulation of NO and SNOs was investigated in tomato plants of the wild type (WT), GSNOR overexpression lines (OE-1/2) and GSNOR suppression lines (AS-1/2) grown under either control conditions or sodic alkaline stress. Phenotype, photosynthesis, reactive oxygen species (ROS) metabolism, Na+-K+ homeostasis and expression of genes encoding ROS scavenging, Na+ detoxification and programmed cell death (PCD) were also analyzed. Compared with WT lines, OE-1/2 lines were alkaline tolerant, while AS-1/2 lines were alkaline sensitive. In AS-1/2 lines, although genetic expression of Na+ detoxification was activated by GSNOR-regulated NO and ROS signaling, excess RNS and ROS accumulation also led to serious oxidative stress and induced PCD. In contrast, overexpression of GSNOR significantly increased ROS scavenging efficiency. Thus, it seemed that increasing alkaline tolerance via GSNOR overexpression in tomato was attributed to the regulation of redox signaling including RNS and ROS.
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