期刊
PLANT AND CELL PHYSIOLOGY
卷 53, 期 2, 页码 485-494出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcs001
关键词
BOR; Boron transporter; Carmenere; Grapes; Parthenocarpic; Vitis
资金
- Fund for the Promotion of Scientific and Technological Development [FONDEF G07I1003]
- Universidad de Talca
- Programa de Mejoramiento de la Calidad y Equidad de la Educacion Superior [MECESUP TAL0601]
- Programas Becas Chile
- Ministry of Education, Culture, Sports, Science and Technology
- Ministry of Agriculture, Forestry and Fisheries of Japan [IPG-0005]
- Grants-in-Aid for Scientific Research [21228002, 23780060] Funding Source: KAKEN
Boron (B) is an essential micronutrient for normal development of roots, shoots and reproductive tissues in plants. Due to its role in the structure of rhamnogalacturonan II, a polysaccharide required for pollen tube growth, B deficiency has been associated with the occurrence of parthenocarpic seedless grapes in some varieties of Vitis vinifera L. Despite that, it is unclear how B is mobilized and accumulated in reproductive tissues. Here we describe the characterization of an efflux B transporter, VvBOR1, homolog to AtBOR1, which is involved in B xylem loading in Arabidopsis thaliana roots. VvBOR1-green fluorescent protein (GFP) fusion protein expressed in A. thaliana localizes in the proximal plasma membrane domain in root pericycle cells, and VvBOR1 overexpression restores the wild-type phenotype in A. thaliana bor1-3 mutant plants exposed to B deficiency. Complementation of a mutant yeast strain indicates that VvBOR1 corresponds to a B efflux transporter. Transcriptional analyses during grapevine reproductive development show that the VvBOR1 gene is preferentially expressed in flowers at anthesis and a direct correlation between the expression pattern and B content in grapes was established, suggesting the involvement of this transporter in B accumulation in grapevine berries.
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