期刊
PLANT AND CELL PHYSIOLOGY
卷 53, 期 12, 页码 2142-2152出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcs155
关键词
OsRecQl4; OsExo1; rice; DSB; HR; resection step
资金
- PROBRAIN (Program for Promotion of Basic Research Activities for Innovative Biosciences) [grant from the Bio-Oriented Technology Research Advancement Institution (BRAIN) of Japan]
- Ministry of Agriculture, Forestry and Fisheries of Japan [Genomics for Agricultural Innovation] [GMC-0001]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [the Strategic Promotion Program for Basic Nuclear Research] [210105, 23658012]
- Grants-in-Aid for Scientific Research [23658012, 23310142] Funding Source: KAKEN
During homologous recombination (HR)-mediated DNA double-strand break (DSB) repair in eukaryotes, an initial step is the creation of a 3'-single-stranded DNA (ssDNA) overhang via resection of a 5' end. Rad51 polymerizes on this ssDNA to search for a homologous sequence, and the gapped sequence is then repaired using an undamaged homologous DNA strand as template. Recent studies in eukaryotes indicate that resection of the DSB site is promoted by the cooperative action of RecQ helicase family proteins: Bloom helicase (BLM) in mammals or Sgs1 in yeast, and exonuclease 1 (Exo1). However, the role of RecQ helicase and exonuclease during the 5'-resection process of HR in plant cells has not yet been defined. Here, we demonstrate that overexpression of rice proteins OsRecQl4 (BLM counterpart) and/or OsExo1 (Exo1 homolog) can enhance DSB processing, as evaluated by recombination substrate reporter lines in rice. These results could be applied to construct an efficient gene targeting system in rice.
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