期刊
PLANT AND CELL PHYSIOLOGY
卷 53, 期 1, 页码 118-124出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcr148
关键词
Phosphoproteomics; Phosphorylation; Plant; Proteomics; Shotgun
资金
- Yamagata Prefecture and Tsuruoka City
- Ministry of Education, Sports, Culture, Science and Technology [21770059, 19678001]
The emergence of 'shotgun proteomics' has paved the way for high-throughput proteome analysis, by which thousands of proteins can be identified simultaneously from complex samples. Although the shotgun approach has the potential to monitor many different post-translational modifications, further technological development is needed to enrich each post-translational 'modificome'. Large-scale in vivo phosphorylation site mapping, so-called shotgun phosphoproteomics, has become feasible in various organisms, including plants, owing to recent technological breakthroughs. Shotgun phosphoproteomics is not a mature technology, but progress has been rapid. In this review, we highlight the scope and limitations of current methods, and some key technological issues in this field.
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