期刊
PLANT AND CELL PHYSIOLOGY
卷 51, 期 12, 页码 2145-2151出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcq161
关键词
Arabidopsis; Methodology; Transcription factor; Yeast one-hybrid screening
资金
- Japan Society for the Promotion of Science (JSPS) [21770064, 20657012, 22687003]
- Grants-in-Aid for Scientific Research [22687003, 20657012, 21770064] Funding Source: KAKEN
Yeast one-hybrid screening is widely used for the identification of transcription factors (TFs) that interact with specific DNA sequences. However, screening a whole cDNA library is not efficient for the identification of TFs because TF genes represent only a small percentage of clones in a cDNA library. Here, we present the development of an efficient yeast one-hybrid screening system using a prey library composed only of approximately 1,500 TF cDNAs of Arabidopsis thaliana. This library enabled us to isolate a TF that binds to a specific promoter sequence with high efficiency, even when the promoter region of the gene of interest was directly employed as bait. Furthermore, this library was also successfully applied as a yeast two-hybrid library to find TFs that interact with specific proteins. This efficient system will contribute to the elucidation of gene regulatory networks in plants.
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