期刊
PLANT AND CELL PHYSIOLOGY
卷 51, 期 2, 页码 328-332出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcp188
关键词
Arabidopsis thaliana; 3 End region; Gene expression; Heat shock protein gene; Polyadenylation; Terminator
资金
- New Energy and Industrial Technology Development Organization
To express a foreign gene in plants effectively, a good expression system is required. Here we describe the identification of a transcriptional terminator that supports increased levels of expression. The terminators of several Arabidopsis genes were examined in transfected Arabidopsis T87 protoplasts. The heat shock protein 18.2 (HSP) terminator was the most effective in supporting increased levels of expression. The HSP terminator increases mRNA levels of both transiently and stably expressed transgenes approximately 2-fold more than the NOS (nopaline synthase) terminator. When combined with the HSP terminator, a translational enhancer increased gene expression levels approximately 60- to 100-fold in transgenic plants.
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