期刊
PLANT AND CELL PHYSIOLOGY
卷 50, 期 4, 页码 904-908出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcp042
关键词
FtsZ1; Fluorescent protein; Male gametophyte; Plastid division; Pollen
资金
- The Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research [16085207, 18870018, 20770036]
- the Oohara Foundation
- Grants-in-Aid for Scientific Research [18870018, 20770036, 16085207] Funding Source: KAKEN
Visualizing organelles in living cells is a powerful method to analyze their intrinsic mechanisms. Easy observation of chlorophyll facilitates the study of the underlying mechanisms in chloroplasts, but not in other plastid types. Here, we constructed a transgenic plant enabling visualization of plastids in pollen grains. Combination of a plastid-targeted fluorescent protein with a pollen-specific promoter allowed us to observe the precise number, size and morphology of plastids in pollen grains of the wild type and the ftsZ1 mutant, whose responsible gene plays a central role in chloroplast division. The transgenic material presented in this work is useful for studying the division mechanism of pollen plastids.
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