期刊
PLANT AND CELL PHYSIOLOGY
卷 49, 期 10, 页码 1407-1416出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcn124
关键词
Anther; Laser microdissection; Microarray; Oryza sativa; L. Microspore/pollen; Tapetum
资金
- The Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) [18075003, 18075012, 18075005, 18075013, 19043015]
- Japan Society for Promotion of Science (JSPS)
- Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF)
- Integrated Research Project for Plant, Insect, and Animal [IPG-0012, IPG-0018]
- Grants-in-Aid for Scientific Research [19043015, 18075005, 18075003, 18075012] Funding Source: KAKEN
In flowering plants, the male gametophyte, the pollen, develops in the anther. Complex patterns of gene expression in both the gametophytic and sporophytic tissues of the anther regulate this process. The gene expression profiles of the microspore/pollen and the sporophytic tapetum are of particular interest. In this study, a microarray technique combined with laser microdissection (44K LM-microarray) was developed and used to characterize separately the transcriptomes of the microspore/pollen and tapetum in rice. Expression profiles of 11 known tapetum specific-genes were consistent with previous reports. Based on their spatial and temporal expression patterns, 140 genes which had been previously defined as anther specific were further classified as male gametophyte specific (71 genes, 51%), tapetum-specific (seven genes, 5%) or expressed in both male gametophyte and tapetum (62 genes, 44%). These results indicate that the 44K LM-microarray is a reliable tool to analyze the gene expression profiles of two important cell types in the anther, the microspore/pollen and tapetum.
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