期刊
PLANETARY AND SPACE SCIENCE
卷 72, 期 1, 页码 138-145出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pss.2012.08.009
关键词
Mars; Biosignatures; Microorganisms; Mass spectrometry; MALDI-TOF-MS; Antarctic glaciers
资金
- Spanish Ministerio de Economia y Competitividad [M/2008-00304/ANT, CTM2010-12134-E/ANT]
Rover missions to Mars require portable instruments that use minimal power, require no sample preparation, and provide suitably diagnostic information to an Earth-based exploration team. In exploration of analog environments of Mars it is important to screen rapidly for the presence of biosignatures and microorganisms and especially to identify them accurately. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) has enormously contributed to the understanding of protein chemistry and cell biology. Without this technique proteomics would most likely not be the important discipline it is today. In this study, besides 'true' proteomics, MALDI-TOF-MS was applied for the analysis of microorganisms for their taxonomic characterization from its beginning. An approach was developed for direct analysis of whole bacterial cells without a preceding fractionation or separation by chromatography or electrophoresis on samples of bacteria from an Antarctic glacier. Supported by comprehensive databases, MALDI-TOF-MS-based identification could be widely accepted within only a few years for bacterial differentiation in Mars analogs and could be a technique of election for Mars exploration. (C) 2012 Elsevier Ltd. All rights reserved.
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