Elevated glucocorticoid metabolism in placental tissue from first trimester pregnancies at increased risk of pre-eclampsia

Background: The local actions of glucocorticoids in the placenta can be modulated by 11 beta-hydroxysteroid dehydrogenase (11 beta HSD) enzymes, which catalyse inter-conversion of cortisol with its inert metabolite, cortisone, and are known to be expressed in the term placenta and decidua. However, the expression and activity of these enzymes have not been well characterised in the first trimester placenta. The aim of this study was to compare 11 beta HSD2 expression and activity in first trimester placental tissue from pregnancies at either relatively low or high risk of developing pre-eclampsia as determined by Doppler ultrasound. Methods: Enzyme expression was assessed by western blot analysis and immunohistochemistry while 11 beta HSD enzyme activities were quantified using radiometric conversion of [3H]-cortisol in the presence of NADP(+) or NAD(+). Results: 11 beta HSD2 was expressed in syncytiotrophoblast of first trimester placenta, and there was no difference in the level of expression of placental 11 beta HSD2 protein between 9 high pre-eclampsia risk and 14 low pre-eclampsia risk pregnancies. NAD(+)-dependent cortisol oxidation was elevated 3-fold in placental tissue from pregnancies at higher risk of pre-eclampsia than in normal pregnancies (50.9 +/- 15.9 versus 18.3 +/- 1.9 pmol cortisone/mg protein.10 min, n = 11 & 12, respectively; P < 0.05). Conclusions: Expression of 11 beta HSD2 is thought to protect the fetus from exposure to maternal cortisol. While other studies have suggested that 11 beta HSD2 is down regulated in term pre-eclamptic placentae, our study suggests that there is increased cortisol inactivation in first trimester placenta prior to week 10 of gestation, from pregnancies at higher risk of developing pre-eclampsia. (C) 2011 Elsevier Ltd. All rights reserved.