期刊
PLACENTA
卷 29, 期 12, 页码 995-1002出版社
W B SAUNDERS CO LTD
DOI: 10.1016/j.placenta.2008.09.010
关键词
Rupture of fetal membranes; PROM; Cytokines; NF-kappa B
资金
- National Health and Medical Research Council (NHMRC) RD Wright Fellowship [454777]
- Melbourne Research Grant Scheme
- NHMRC Project grant [367615]
- ANZ Charitable Trust (Medical Research and Technology Grant)
- Medical Research Foundation for Women and Babies
To generate new insights into the process of fetal membrane rupture, we have developed a technique whereby fetal membranes overlying the cervix (i.e. supracervical site, SCS) are tagged in women undergoing term elective Caesarean section. The specific aim is to determine the effect of supracervical apposition on the release of inflammatory mediators and NF-kappa B signalling in pre-labour fetal membranes. Fetal membranes were collected from both the SCS and from a distal site (DS). The level of NF-kappa B proteins and its transcriptional co-activator protein CBP and p300 was determined by Western blotting and/or immunohistochemistry (IHC), and cytokine and prostaglandin release was quantified by enzyme immunoassay. Tissues obtained before labour at term possess an area of the fetal membranes (i.e. supracervical site) that exhibit decreased release of IL-1 beta, IL-6, IL-8, TNF-alpha. and PGE(2). IHC revealed that NF-kappa B signalling proteins, CBP and p300 were significantly increased in SC fetal membranes compared to distal membranes. In summary, data from this study confirm that supracervical fetal membranes display altered structural and biochemical characteristics. (c) 2008 Elsevier Ltd. All rights reserved.
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