Modulation of Radiation-Induced Alterations in Oxidative Stress and Cytokine Expression in Lung Tissue by Panax Ginseng Extract

We investigated the modulating effect of Panax ginseng extract (PGE) on radiation-induced lung injury (RILI) by measuring early changes in oxidative stress levels, cytokine expression, and the histopathology of mouse lung tissue treated with high dose of X-ray radiation. The mice were pretreated with 25, 50, and 100-mg/kg doses of PGE orally for four consecutive days, and their thoraces were then exposed to 15-Gy X-ray radiation 1h after the last administration of PGE on day 4. The pretreatments with 50 and 100mg/kg PGE led to significant reductions in the elevation of lipid peroxidation levels at 2 and 10days, respectively, after irradiation. The mice pretreated with PGE exhibited dose-dependent reductions in the irradiation-induced production of tumor necrosis factor and transforming growth factor 1 cytokines 10days after irradiation, with these reductions nearly reaching the control levels after the 100-mg/kg dose. Furthermore, together with providing significant protection against reductions in catalase activity and glutathione content, pretreatment with 100mg/kg PGE resulted in a marked attenuation of the severity of inflammatory changes in lung tissue 10days after irradiation. A high pretreatment dose of PGE may be a useful pharmacological approach for protection against RILI. Copyright (c) 2014 John Wiley & Sons, Ltd.