期刊
PHYTOTHERAPY RESEARCH
卷 28, 期 1, 页码 62-68出版社
WILEY-BLACKWELL
DOI: 10.1002/ptr.4954
关键词
airway mucin; MUC5AC; wogonin; NF-B
In this study, we investigated whether wogonin significantly affects MUC5AC mucin gene expression and production in human airway epithelial cells. Confluent NCI-H292 cells were pretreated with wogonin for 30min and then stimulated with tumor necrosis factor- (TNF-) for 24h or the indicated periods. The MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA, respectively. We found that incubation of NCI-H292 cells with wogonin significantly inhibited mucin production and down-regulated MUC5AC gene expression induced by TNF- in a dose-dependent fashion. To elucidate the action mechanism of wogonin, effect of wogonin on TNF--induced NF-B signaling pathway was investigated by western blot analysis. Wogonin inhibited NF-B activation induced by TNF-. Inhibition of IKK by wogonin led to the suppression of IB phosphorylation and degradation, p65 nuclear translocation and NF-B-regulated gene expression. This, in turn, led to the down-regulation of MUC5AC protein production in NCI-H292 cells. Wogonin also inhibited the gene products involved in cell survival (Bcl-2) and proliferation (cyclooxygenase-2). These results suggest that wogonin inhibits the NF-B signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production. Copyright (c) 2013 John Wiley & Sons, Ltd.
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