期刊
PHYTOTHERAPY RESEARCH
卷 28, 期 4, 页码 586-592出版社
WILEY
DOI: 10.1002/ptr.5034
关键词
sesquiterpene lactone; c-Fos; osteoclast; osteoporosis; costunolide
资金
- Korean Health Technology R&D Project. Ministry of Health and Welfare, Republic of Korea [A120152]
Costunolide, a sesquiterpene lactone, exhibits anti-inflammatory and anti-oxidant properties and mediates apoptosis. However, its effects and mechanism of action in osteoclasts remain unknown. Herein, we found that costunolide significantly inhibited RANKL-induced BMM differentiation into osteoclasts in a dose-dependent manner without affecting cytotoxicity. Costunolide did not regulate the early signaling pathways of RANKL, including the mitogen-activated protein kinase and NF-kappa B pathways. However, costunolide suppressed nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) expression via inhibition of c-Fos transcriptional activity without affecting RANKL-induced c-Fos expression. The inhibitory effects of costunolide were rescued by overexpression of constitutively active (CA)-NFATc1. Taken together, our results suggest that costunolide inhibited RANKL-induced osteoclast differentiation by suppressing RANKL-mediated c-Fos transcriptional activity. Copyright (c) 2013 John Wiley & Sons, Ltd.
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