期刊
PHYTOTHERAPY RESEARCH
卷 26, 期 8, 页码 1211-1219出版社
WILEY-BLACKWELL
DOI: 10.1002/ptr.3705
关键词
apoptosis; cryptotanshinone; mitochondria; Salvia miltiorrhiza; Salviae miltiorrhizae radix; sodium nitroprusside
资金
- Ministry of Health, Welfare and Family Affairs [B100049]
- research fund of Studies on the Identification of the Efficacy of Biologically Active Components from Oriental Herbal Medicines from Korean Food and Drug Administration, Republic of Korea
- Oriental Medicine RD Project
- Korea Health Promotion Institute [B100049] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The root of Salvia miltiorrhiza (Salviae miltiorrhizae radix), a herbal medicine has widely been used for the treatment of pain, miscarriage and oedema. In this study, we evaluated the neuroprotective effect of cryptotanshinone (CRT) from Salviae miltiorrhizae radix on sodium-nitroprusside (SNP)-induced apoptosis in neuro-2a (N2a) cells, and further investigated its action mechanism in signalling pathways. The effects of CRT against SNP-induced toxicity, mitochondrial membrane potential (MMP) changes, and oxidants/antioxidant defences and apoptotic signalling pathways were investigated in N2a cells. Cryptotanshinone significantly inhibited SNP-induced cell toxicity and the generation of reactive oxygen and nitrogen species (RONS), and improved MMP in N2a cells. Cryptotanshinone significantly suppressed SNP-induced peroxidation of lipid and protein, and the expression of Gclc mRNA. In the signalling pathway, CRT effectively blocked SNP-induced activation of NF-?B and ERK1/2 and JNK MAPK pathways through the elevation of Akt and cyclic AMP response element binding protein. Furthermore, CRT remarkably reduced the increase of mitochondrial Bax/Bcl-2 ratio, the release of cytochrome c from mitochondria to cytosol, and the activations of cytosolic procaspase-3 and nuclear inactive poly ADP (adenosine diphosphate)-ribose polymerase by SNP-induced apoptosis. These results indicate that CRT has neuroprotective effects against SNP-induced apoptosis in neuronal cells via the regulation of mitochondrial apoptotic cascades and antiapoptotic cellular signalling pathways. Copyright (c) 2012 John Wiley & Sons, Ltd.
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