Breakdown of Host Resistance by Independent Evolutionary Lineages of Beet necrotic yellow vein virus Involves a Parallel C/U Mutation in Its p25 Gene

Breakdown of sugar beet Rz1-mediated resistance against Beet necrotic yellow vent writs (BNYVV) infection was previously found, by reverse genetics. to be caused by a single Initiation in its p25 gene. The possibility of alternative breaking mutations. however. has not been discarded To explore the natural diversity of BNYVV in the field and its effects on overcoming Rz1. wild-type (WT) and resistance-breaking (RB) p25 genes from diverse production re-ions of North America Were characterized The relative titer of WT p25 was Inversely correlated with disease expression in Rz1 plants from Minnesota and California in Minnesota, the predominant WT p25 encoded the A(67)C(68) amino acid signature whereas. in California. it encoded A(67)L(68) In both locations, these WT signatures were associated with asymptomatic BNYVV infections of Rz1 cultivars Further analyses of symptomatic resistant plains revealed that. in Minnesota, WT A67C68 was replaced by V67C68 whereas. In California. WT A(67)L(68) was replaced by V67L68 Therefore V-67 was apparently critical in overcoming RZ1 in both pathosystems The greater genetic distances between Isolates from different geographic regions rather than between WT and RB from the same location indicate that the Underlying C to U transition originated independently in both BNYVV lineages.