期刊
PHYTOPATHOLOGY
卷 99, 期 12, 页码 1355-1364出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PHYTO-99-12-1355
关键词
asexual recombination; chromosomal exchange; somatic recombination
资金
- Agriculture and Agri-Food Canada
- NSERC
Vegetative or parasexual recombination is thought to be a key mechanism for the genetic diversity of cereal rust fungi. The process of germ tube fusion leading to hyphal anastomosis and nuclear recombination was analyzed in wheat leaf rust fungus, Puccinia triticina. Germ tube anastomosis was observed in 27 P. triticina isolates, each representing a different virulence phenotype. Germ tube fusion bodies (GFBs), which appeared as viscid globules formed at tips of germ tubes, were essential for germ tube anastomosis. The formation of GFBs was affected by the urediniospore density and the length of illumination during germination. GFBs were formed at the highest frequency when urediniospores were spread to a concentration of 1 x 10(6) urediniospores/ml and incubated in dark for 12 to 24 h during germination. GFB attached to either the side of another germ tube ( tip to side) or to another GFB formed at the tip of a second germ tube (tip to tip). In tip to side anastomosis, two nuclei in the germ tube bearing the GFB migrated into the second germ tube through the GFB which resulted in four nuclei within this germ tube. In tip to tip anastomosis, nuclei in both germ tubes migrated into the fused GFB and all four nuclei came into close proximity. Urediniospores of isolates MBDS-3-115 and TBBJ-5-11 were stained with DAPI (4',6'diamine-2-phenylindole) and Nuclear Yellow (Hoechst S769121), respectively, and then mixed and germinated on water agar. Some fused GFBs contained nuclei stained with DAPI and nuclei stained with Nuclear Yellow in close proximity, demonstrating the fusion between genetically different P. triticina isolates. In some fused GFBs, bridge-like structures connecting different nuclei were observed.
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